genortГений ортопедииGenij Ortopedii1028-44272542-131XЦЕНТР ИЛИЗАРОВА10.18019/1028-4427-2024-30-6-881-888UZZAICgenort-3117Research ArticleОригинальные статьиOriginal articlesЛокализация остеокальцина в процессе заживления костной ткани при местном применении коллагена и бета-трикальцийфосфата у крысLocalization of osteocalcin in bone healing treated by local application of collagen and beta-tricalcium phosphate in ratshttps://orcid.org/0009-0007-5944-3868HusseinB. J.HusseinB. J.

Bayan Jabr Hussein — PhD, Assistant Professor, Lecturer

Baghdad

Bayan Jabr Hussein — PhD, Assistant Professor, Lecturer

Baghdad

medicalresearch79@yahoo.comhttps://orcid.org/0009-0000-7542-3858GhaniB. A.GhaniB. A.

Ban А. Ghani — PhD, Professor, Head of Department

Baghdad

Ban А. Ghani — PhD, Professor, Head of Department

Baghdad

drban871961@gmail.comCollege of Dentistry, University of BaghdadИракCollege of Dentistry, University of BaghdadIraq202418122024306881888Copyright © Hussein B.J., Ghani B.A., 20242024Hussein B.J., Ghani B.A.Hussein B.J., Ghani B.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.ilizarov-journal.com/jour/article/view/3117Введение

Введение. Восстановление костной ткани — сложный и многогранный процесс, который происходит естественным образом, если не осложняется значительными дефектами кости. Бета-трикальцийфосфат (β-TCP) известен своими богатыми запасами кальция и фосфора, легко усваиваемыми организмом. Его  исключительная биосовместимость способствует формированию рассасывающейся структуры в зоне повреждения, активизируя процесс заживления.

Цель работы — определение влияния скаффолда из коллагена/β-трикальцийфосфата (Coll/β-TCP) на формирование костной ткани для оценки возможности его дальнейшего использования в качестве вспомогательного средства для возмещения дефектов кости.

Материалы и методы

Материалы и методы. Эксперимент проведен на 20 взрослых самцах крыс-альбиносов. У каждого животного в условиях операционной создано по два отверстия в каждой бедренной кости; два отверстия обрабатывали отдельно Coll или β-TCP, одно отверстие — их комбинацией. Необработанное отверстие служило контролем. Скарификацию животных проводили после двух- и четырехнедельного периода лечения (по 10 крыс на каждый). Проведен иммуногистохимический анализ стромальных клеток костного мозга, остеоцитов, остеобластов и остеокластов с помощью поликлональных антител к остеокальцину.

Результаты

Результаты. Иммуногистохимические результаты выявили сильную положительную экспрессию остеокальцина при заживлении костей в комбинированной группе (β-TCP и коллаген) по сравнению с  другими группами. Высоко значимые различия были обнаружены между группой с комбинацией коллагена с β-TCP и контрольной группой на обоих сроках эксперимента.

Обсуждение

Обсуждение. Маркер остеокальцина уникален для остеобластов, в частности для остеобластов, активно формирующих новый остеоид или ремоделирующих кость. Полученные результаты показали, что экспрессия остеокальцина была выше в опытных группах, чем в контрольной.

Заключение

Заключение. Комбинация коллагена с β-TCP показала наибольшую эффективность ускорения заживления костной ткани и увеличения остеогенной способности за счет повышенной иммунореактивности остеокальцина.

Introduction Bone repair is a complex and multifaceted process that generally happens naturally unless complicated by situations such as substantial bone defects. The bone healing process is typically divided into three stages: inflammation, repair, and remodeling. Beta-tricalcium phosphate (β-TCP) renowned for its abundant reserves of calcium and phosphorus, easily assimilated by the body. Its exceptional biocompatibility assists in the formation of an absorbable interlinked structure at the injury site, contributing to the advancement of the healing process.

Purpose This study aimed to estimate the effects of a scaffold of collagen/β-tricalcium phosphate (Coll/βTCP) on bone construction to evaluate its latent usage as a bone auxiliary to repair bone defects.

Material and Methods The experiment was performed on 20 adult male albino rats. Four holes were surgically created on each animal, two in each femur; two holes were treated separately with Coll or β-TCP, one hole with their combination. The untreated hole served as a control. Animals were scarified after twoand four-week treatment periods (10 rats for each). Immunohistochemical analysis of bone marrow stromal cells, osteocytes, osteoblasts and osteoclasts using polyclonal antibodies to osteocalcin was performed.

Result Immunohistochemical results discovered strong positive expression of osteocalcin in bone healing in the group of combined treatment (β-TCP and collagen) as compared to other groups. Highly significant differences were seen between the combination of collagen with β-TCP and the control group at both timepoints of the experiment.

Discussion The marker osteocalcin is unique to osteoblasts, specifically to osteoblasts that are actively forming new osteoid or remodeling bone. The obtained findings showed that mean values of osteocalcin expression were greater in the experimental groups than in the control group.

Conclusion The combination of collagen with β-TCP showed the greatest efficacy in accelerating bone healing and increasing osteogenic capacity due to increased osteocalcin immunoreactivity.

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The authors declare that there are no conflicts of interest present.