genortГений ортопедииGenij Ortopedii1028-44272542-131XЦЕНТР ИЛИЗАРОВА10.18019/1028-4427-2023-29-2-190-203PIOJYRgenort-2837Research ArticleОригинальные статьиOriginal articlesМодели создания гнойно-септического воспаления большеберцовой кости у крысы для оценки действия биорезорбируемых материалов с антимикробными препаратамиModels for purulent septic inflammation of the tibia in rats to assess the effect of bioresorbable materials with antimicrobial drugshttps://orcid.org/0000-0001-5386-1929СмоленцевД. В.SmolentsevD. V.

Дмитрий Владимирович Смоленцев – научный сотрудник

Москва

Dmitry V. Smolentsev

Moscow

SmolentsevDV@cito-priorov.ruhttps://orcid.org/0000-0003-0121-1232ЛукинаЮ. С.LukinaYu. S.

Юлия Сергеевна Лукина – кандидат технических наук, старший научный сотрудник

Москва

Yulia S. Lukina – Candidate of Technical Sciences

Moscow

lukina_rctu@mail.ruhttps://orcid.org/0000-0002-1326-6794Бионышев-АбрамовЛ. Л.Bionyshev-AbramovL. L.

Леонид Львович Бионышев-Абрамов – оператор ЭВМ

Москва

Leonid L. Bionyshev-Abramov

Moscow

sity-x@bk.ruhttps://orcid.org/0000-0002-4097-1552СережниковаН. Б.SerezhnikovaN. B.

Наталья Борисовна Сережникова – кандидат биологических наук, старший научный сотрудник

Москва

Natalya B. Serezhnikova – Candidate of Biological Sciences

Moscow

natalia.serj@yandex.ruhttps://orcid.org/0000-0001-9810-6513ВасильевМ. Г.VasilievM. G.

Максим Геннадьевич Васильев – кандидат медицинских наук, старший научный сотрудник

Москва

Maxim G. Vasiliev – Candidate of Medical Sciences

Moscow

maxox@mail.ruhttps://orcid.org/0000-0002-4981-0149СенягинА. Н.SenyaginA. N.

Александр Николаевич Сенягин – исследователь

Москва

Aleksandr N. Senyagin

Moscow

senyagin_an@pruf.ruhttps://orcid.org/0000-0003-4900-3555ПхакадзеТ. Я.PkhakadzeT. Ya.

Тамара Яковлевна Пхакадзе – доктор медицинских наук, заведующий лабораторией

Москва

Tamara Ya. Pkhakadze – Doctor of Medical Sciences

Moscow

microlab_cito@mail.ruНациональный медицинский исследовательский центр травматологии и ортопедии имени Н.Н. ПриороваРоссияPriorov National Medical Research Center of Traumatology and OrthopaedicsRussian FederationПервый Московский государственный медицинский университет имени И.М. Сеченова (Сеченовский Университет)РоссияSechenov First Moscow State Medical UniversityRussian FederationРоссийский университет дружбы народовРоссияPeoples’ Friendship University of RussiaRussian Federation202328042023292190203Copyright © Смоленцев Д.В., Лукина Ю.С., Бионышев-Абрамов Л.Л., Сережникова Н.Б., Васильев М.Г., Сенягин А.Н., Пхакадзе Т.Я., 20232023Смоленцев Д.В., Лукина Ю.С., Бионышев-Абрамов Л.Л., Сережникова Н.Б., Васильев М.Г., Сенягин А.Н., Пхакадзе Т.Я.Smolentsev D.V., Lukina Y.S., Bionyshev-Abramov L.L., Serezhnikova N.B., Vasiliev M.G., Senyagin A.N., Pkhakadze T.Y.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.ilizarov-journal.com/jour/article/view/2837Введение

Введение. Приведен краткий обзор моделей создания гнойно-септического воспаления у крыс, в том числе с помощью активного бактериального агента и методы диагностики воспаления.

Цель

Цель. Демонстрация результатов разработки эффективной экспериментальной модели создания гнойно-септического воспаления большеберцовой кости крысы с использованием малоинвазивных методов диагностики заражения in vivo.

Материалы и методы

Материалы и методы. На четырех группах мелких лабораторных животных исследованы различные модели создания гнойно-септического воспаления при применении инокуляции золотистого стафилококка. Отработаны не разрушаемые объект методы оценки гнойно-септического воспаления: микробиологическая, томографическая, морфологическая.

Результаты

Результаты. Результаты исследования свидетельствуют о возможности создания экспериментального гнойно-септического воспаления у крыс к 14-60 суткам с помощью инокуляции S. aureus, которое представляет собой тяжелую, быстро прогрессирующую гнойную инфекцию, приводящую к обширному разрушению кости с образованием секвестров.

Обсуждение

Обсуждение. Для гарантированного формирования гнойно-воспалительного процесса костной ткани в более короткие сроки наблюдения необходима контролируемая в количественном отношении инвазия активного бактериального агента. Склерозирующий агент и формирование свищевого хода не являются обязательными при создании воспаления.

Заключение

Заключение. Продемонстрированы результаты разработки экспериментальных моделей создания гнойно-септического воспаления с применением малоинвазивных методов диагностики in vivo, что позволит получить адекватную оценку степени инфицирования перед лечением.

Introduction A brief review of modeling purulent septic inflammation in rats, including with the help of an active bacterial agent, and methods for diagnosing inflammation are given.

The aim of the study was to demonstrate the results of the development of an effective experimental model of purulent septic inflammation of the tibia in rats using minimally invasive methods for diagnosing infection in vivo.

Materials and methods Various models of purulent septic inflammation were studied in four groups of small laboratory animals, when using the inoculation of Staphylococcus aureus. Methods for assessing purulent-septic inflammation that are not destructible by the object have been worked out: microbiological, tomographic, morphological.

Results The results of the study indicate the possibility of creating experimental purulent-septic inflammation in rats by 14-60 days using S. aureus inoculation, which is a severe, rapidly progressive purulent infection that leads to extensive destruction of the bone with the formation of sequesters.

Discussion To guarantee the formation of a purulent-inflammatory process of bone tissue in a shorter period of observation, a quantitatively controlled invasion of an active bacterial agent is necessary. A sclerosing agent and formation of a fistulous tract are not essential in creating inflammation.

Conclusion The results of the development of experimental models for the creation of purulent-septic inflammation using minimally invasive in vivo diagnostic methods are demonstrated, which will allow an adequate assessment of the degree of infection before treatment.

остеомиелитгнойно-септическое воспалениебактериальная инвазиязолотистый стафилококксклерозирующий агентинокуляцияКОЕosteomyelitispurulent-septic inflammationbacterial invasiongolden Staphylococcus aureussclerosing agentinoculationCFUИсследование выполнено в рамках государственного задания ФГБУ «НМИЦ ТО им. Н.Н. Приорова» Минздрава России «Разработка технологии получения остеопластических полимерных и кальций-фосфатных материалов с регулируемой скоростью высвобождения антибиотиков и целевых фармацевтических субстанций для оперативного лечения гнойных процессов костной ткани и профилактики формирования бактериальных биопленок на имплантируемых металлоконструкциях».ReferencesLew DP, Waldvogel FA. Osteomyelitis. Lancet. 2004;364(9431):369-79. doi: 10.1016/S0140-6736(04)16727-5Lew DP, Waldvogel FA. Osteomyelitis. Lancet. 2004;364(9431):369-79. doi: 10.1016/S0140-6736(04)16727-5Inzana JA, Schwarz EM, Kates SL, Awad HA. Biomaterials approaches to treating implant-associated osteomyelitis. 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The authors declare that there are no conflicts of interest present.